trials

LIFE CLONING GEL & SOLUTION TRIAL (2015)

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PURPOSE

LIFE cloning gel & solution trial documenting root time and quality over a 14 day period

PICTURES

Day 1

Day 1

Day 5

Day 9

Day 14

Day 14

 


This trial was performed at Rx Green Technologies’ testing facility in Denver, CO. Individual results may vary.

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LIFE CLONING SOLUTION TRIAL (2014)

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ROOTING OF BASIL STEM CUTTINGS IN RESPONSE TO CLONING SOLUTIONS

Data presented by: Neil Mattson, Associate Professor/Dept. of Horticulture, Cornell University

PURPOSE

To compare the effect of two different cloning solutions, LIFE and Clonex, on the formation and development of roots in basil stem cuttings propagated in aeroponics.

SUMMARY

Roots systems for LIFE cuttings had on average 20% FASTER ROOTS, 23% MORE ROOTS (dry weight basis) and 32% LONGER ROOTS than Clonex cuttings.

PROTOCOL

Stock plants of basil (Ocimum basilicum) ‘Pluto’ were grown in 4-inch containers in a peat/perlite based soilless substrate (Lambert LM-111). Stock plants were grown and the experiment was conducted in glass greenhouse at Cornell University (Ithaca, NY) maintained at 70 °F. Ambient light was supplemented with 1000 W high pressure sodium lamps suspended about 5 feet above plant canopy delivering ample light daily.

Two cloning solutions were compared in the experiment LIFE (0.5-1-0.5) and Clonex (1-0.4-1). Eight aeroponic units (Botanicare Clone Machine 48) were used for rooting. Four units were randomly selected for use with LIFE and 4 with Clonex solutions. Each unit was filled with 8 gallons of reverse osmosis purified water. Cloning solutions were added following their label rates. The pH of the solution was adjusted to 5.5-5.7.

Cuttings were inserted into neoprene collars (with 1 cutting per collar) and placed into aeroponic units with about 4 cm of each cutting below the collar. For each rooting date, 6 basil cuttings were installed in each aeroponic unit. Every 2-3 days another series of cuttings was selected for rooting for a total of four rooting dates. Each cutting was monitored every 2-3 days to take pictures of representative plants and to record visual root index. When cuttings were monitored, cloning solution was added to top off the containers to the 8 gallon fill line. At this time pH and electrical conductivity (EC) were recorded and pH was adjusted to 5.5-5.7. Every 7 days the cloning solution was completely replaced.

Within each series of cuttings, measurements were taken to determine root index, length of the longest root of each cutting, and roots were removed from the stem and placed in an oven to determine dry weight.

4 series of cuttings (2 x 4 x 6 x 4 = 192). For either cloning solution no cuttings were observed to have died during the experiment.

FINDINGS

Roots appeared on stem cuttings about 4-5 days after rooting. LIFE cuttings had visually longer roots than Clonex and were ready for transplant at day 7-8 while Clonex cuttings were ready for transplant on day 9-10.

“Basil cuttings propagated using LIFE were ready for transplant about two days earlier than Clonex cuttings.” –Professor Neil Mattson

The length of the longest root on each stem cutting was measured at day 9-10. Root length can be taken as an overall quality measurement of the root system. In each of the 4 rooting series there was a significant increase in root length for LIFE cuttings as compared to Clonex.

“Overall, cuttings propagated with LIFE cloning solution had a 32% greater root length than Clonex” –Professor Neil Mattson

Root dry weight was determined by separating roots from stem cuttings at day 9-10. Dry weight is a good measure of the total biomass (or overall size) of the root system. Life cuttings had a 23% greater root dry weight than Clonex.

CONCLUSION

For all three measured parameters of root quality (index, length, and dry weight), the basil cuttings propagated aeroponically with LIFE cloning solution were superior to those propagated using Clonex cloning solution.

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LIFE CLONING GEL VS INDUSTRY LEADER (2014)

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SUMMARY

After over 12 months of research and development, we finally found the ultimate formula for our LIFE Gel product. We are pretty excited about the results from this Liquid Gold formula. To make it easy, there is no need to change your cloning process….cut, dip and place into a plug or aeroponic machine. Water every few days and the results will be impressive.

What is so interesting about LIFE Gel? Similar to other cloning gels, LIFE Gel contains IBA and B1 Vitamin to promote root development. What is unlike other cloning gels is LIFE Gel contains a naturally occurring biocide, Thyme Oil, which replaces the standard ingriedient 1,2-benzisothiazol-3(2H)-one, which is known health and environmental concerns and require EPA regulation. Not only does the product contain natural ingredients, LIFE Gel just plain works better than the competition.

PICTURES

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CLEAN COCO Substrate

Clean Coco Trial

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MEASURING GROWTH, YIELD, AND THC POTENCY

Data presented by: Stephanie Wedryk, PhD, Director of R&D at Rx Green Technologies

PURPOSE

To compare the effect of two different coconut coir substrate products, CLEAN COCO and a leading competitor (Competitor Coco), on cannabis growth, yield, and potency.

PROTOCOL

First, CLEAN COCO and Competitor Coco were chemically tested at Midwest Laboratories, a Colorado State approved laboratory.

CHEMICAL COMPARISON OF COMPETITOR AND CLEAN COCO

Competitor Coco Clean Coco
pH 7.1 7.0
Soluble Salts/EC 1.2 0.28
P 12 <10
K 100 50
Mg <10 <10
Na 82 23
Ca <10 <10
CEC 24 32

Higher CEC (Cation Exchange Capacity) indicates greater ability to hold certain nutrients (K, Ca, Mg, Mn, Fe, Zn) with lower potential to lose them in runoff. CLEAN COCO has a higher CEC than Competitor Coco, ensuring greater nutrient availability. Additionally, Na in high concentrations can be toxic to plants. Competitor Coco has a higher concentration of Na than CLEAN COCO.

Next, the cannabis variety Quattro Kush was planted in CLEAN COCO and Competitor Coco. Clones were transplanted directly into coconut coir substrate and tracked through harvest for differences in growth, yield, and potency

GROWTH: Stem diameter and plant height were measured weekly upon transplant of clones until the fifth week of the flowering cycle. Canopy diameter was measured weekly from clone transplant through the last week of the vegetative cycle.

YIELD: Bud weight was measured per Colorado State compliance regulations, both directly after being harvested and trimmed, and after drying.

POTENCY: THC was measured at an approved Colorado State laboratory.

FINDINGS

GROWTH

Stem Diameter was statistically greater in CLEAN COCO treatments than Competitor Coco during the flowering cycle.

Height of Quattro Kush in CLEAN COCO and Competitor Coco was statistically equivalent.

Canopy of Quattro Kush grown in CLEAN COCO and Competitor Coco was statistically equivalent.

YIELD

Quattro Kush grown in CLEAN COCO yielded more bud weight per plant.

POTENCY

THC in Quattro Kush was higher when grown in CLEAN COCO.

CONCLUSION

Quattro Kush grown in CLEAN COCO had increased growth, yields, and potency compared to the Competitor Coco. CLEAN COCO has the potential to improve cannabis yield and potency compared to competitor brands. Our team believes that the combination of low salts and high CEC via strict quality controls led to the outperformance via the use of CLEAN COCO.

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NOTICE: Always read and follow product label directions. Rx Green Technologies’ products can be used to promote and facilitate the growth of a variety of agricultural products and plants including, without limitation, cannabis. Rx Green Technologies does not own, distribute or sell cannabis. The research facility affiliated with Rx Green Technologies is fully compliant with all applicable state and local code. Please consult your local or state laws regarding what plants may lawfully be grown in your state.